You would like to cultivate Escherichia coli in a chemostat culture. The maximum specific growth rate (?max) of E. coli that you can reach is known as 1.0 h?Ā¹ at your culture conditions. Describe what you would observe for each condition if you have the following settings: F (flow rate of the fresh medium into the bioreactor vessel) (L/h) | V (Volume of the liquid culture in the bioreactor vessel) (L) a) 1 | 1 b) 1 | 0.8 c) 0.5 | 1 Hint: Dilution rate (D) = F ? V
Added by Robert C.
Close
Step 1
** Show moreā¦
Show all steps
Your feedback will help us improve your experience
Adi S and 85 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
A certain strain of E. Coli grows with a doubling time of 0.5 h in the exponential growth phase. a) Determine the maximum specific growth rate. b) Calculate the amount of time that would be required to grow the cell culture for 0.1 kg m-3 to 10 kg m-3. Assume the culture is in the exponential growth phase throughout this time.
Adi S.
a. During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b. If you plated a 100Ī¼L of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect to see on a plate? Show and explain your calculations.
Jenny W.
You diluted a starting culture of E. coli 1:10 ten times. From these dilutions you plated 0.1mL of each dilution onto an agar plate. You then counted the number of bacterial colonies that grew after incubation (see below). Give the average estimated starting concentration of bacteria per milliliter from the plates shown below. Don't forget units! Show your work. 1 x 10^-9 dilution (1/1,000,000,000) 1 x 10^-10 dilution (1/10,000,000,000)
Shaiju T.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD