You inoculated one phenol red broth (containing fructose) with E. coli. After incubating at 25°C for 48 hours, you get the following result (right). What can you conclude about this organism based on these results? Be sure to explain how you are able to come to your conclusion. An uninoculated control tube (left) is provided as a comparison.
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First, we need to understand what phenol red broth is and what it contains. Phenol red broth is a liquid medium that contains a pH indicator called phenol red. It also contains a carbohydrate source, in this case, fructose, which can be used by bacteria as a Show more…
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Phenol Red Broth Your Results and Observations Record your results after incubation in the table.
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Lab name: Phenol Red Broth. (please be detailed with. your answer) Early formulations of this medium used a smaller amount of carbohydrate and occasionally produced false alkaline (pink) results after 48 hours. Why do you think this happened? List at least 2 steps, as a microbiologist, you could take to prevent the problem. Suppose you inoculate a PR broth with a slow-growing fermenter. After 48 hours, you see slight turbidity but score (-/-). Is this a false positive or a false negative. Is this failure a result of a lack of specificity or sensitivity of the test system?
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