Question

Your PCR that was discussed above (the gene that would make cells resistant to certain mutations) was successful, but to make sure, you need to run a sample of your DNA via gel electrophoresis to confirm. However, a colleague of yours requires some lanes on your gel to run their own samples at the same time. You load samples of your product into two lanes of the gel, and your colleague uses six other lanes. The initial lane (Lane 1) contains a 100 base pair molecular weight ladder with band sizes of 1000, 900, 800, 700, 600, 500, 400 (the brightest band), 300, 200, 100, and 20 base pairs respectively. Below is the image taken of your gel after running the gel at 130V for 30 minutes: Based on what you know about your sequence of interest, which lanes contain your DNA sample?

          Your PCR that was discussed above (the gene that would make cells resistant to certain mutations) was successful, but to make sure, you need to run a sample of your DNA via gel electrophoresis to confirm. However, a colleague of yours requires some lanes on your gel to run their own samples at the same time. You load samples of your product into two lanes of the gel, and your colleague uses six other lanes. The initial lane (Lane 1) contains a 100 base pair molecular weight ladder with band sizes of 1000, 900, 800, 700, 600, 500, 400 (the brightest band), 300, 200, 100, and 20 base pairs respectively. Below is the image taken of your gel after running the gel at 130V for 30 minutes: Based on what you know about your sequence of interest, which lanes contain your DNA sample?

Added by Concepci-N M.

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