Your team has been given the following gene with the sequence. This gene consists of three exons (uppercase) and two introns (lowercase). A messenger RNA is generated from the complementary strand (template strand). The mRNA will consist of noncoding and a coding region. 5'-ATG AAG GTA TAG CAG GTT TTA GTA CAG TAG-3
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First, we need to identify the exons and introns in the gene sequence. Exons are the coding regions of the gene, while introns are the noncoding regions. The gene sequence is: 5'-ATG AAG GTA TAG CAG GTT TTA GTA CAG TAG-3' The exons are represented by uppercase Show more…
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GENE EXPRESSION - Breaking the code TRANSCRIPTION For each of the same DNA sequences below, write the sequence of messenger RNA codons that is synthesized during transcription. Be sure to separate the RNA into codons. DNA molecule #1: TAC CGG ATG CCA GAT CAA ATC mRNA #1 DNA molecule #2: TAC GGG GGC GTA ACC ACA ACT mRNA #2 TRANSLATION For each of the mRNA codon sequences you have written, determine the sequence of tRNA anticodons that match it. tRNA (anticodons) for mRNA #1: tRNA (anticodons) for mRNA #2: Using the table on the next page, write the amino acid sequence (polypeptide) coded for by each mRNA. (Note: The code is based on mRNA codons, not tRNA anticodons.) Polypeptide #1: Polypeptide #2:
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Write the mRNA of the following coding strand of the DNA (5 pts): DNA: TAC-AAA-TTT-CCC-GGG-ACT mRNA: ? Translate the above mRNA by using table of universal genetic code (5pts): Protein: ?
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3. You have a segment of DNA that contains the following sequence: -----------5'-GGACTAGACAATAGGGACCTAGAGATTCCGAAA-3'----------- -----------3'-CCTGATCTGTTATCCCTGGATCTCTAAGGCTTT-5'----------- You know that the RNA transcribed from this segment contains the following sequence: 5'-GGACUAGACAAUAGGGACCUAGAGAUUCCGAAA-3' a) Does the top strand or the bottom strand serves as the template for synthesis? b) On the DNA sequence, draw an arrow indicating the +1 site. 4. The sequence of the middle of a primary transcript is shown below. Exons are shown in green; introns are shown in purple. 5'-GTCAGACGTACGTCAGTCAGGUACACCACTGACCCATTGAGGCACTCGACC-3' a) Write the sequence of this region in the mature mRNA. b) Write the sequence of amino acids that will be synthesized from this region of mRNA. The reading frame begins with the first base at the appropriate end of the strand.
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