You're feeding your E. coli culture when the old labels on your media bottles fall off causing you to accidently feed with a media containing lactose but no glucose. What happens to the transcriptional regulation of the lac operon in these bacteria?
Added by Guillermo W.
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coli uses it as a preferred energy source. The presence of glucose inhibits the activity of the cAMP (cyclic AMP) receptor protein (CRP), also known as the catabolite activator protein. CRP is a transcriptional activator that, when bound to cAMP, activates the Show more…
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You're growing a culture of E. coli cells without glucose that has a mutation in the lac operator sequence such that it can't bind the lac repressor. Explain what would be occurring to the Lac operon in the presence of lactose. Explain what would be occurring in the absence of lactose.
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