Here is the sequence of a portion of a bacterial gene:
2'GTATCGTATGCATGCATCGTGAC3'
3'CATAGCATACGTACGTAGCACTG
The template strand is on the bottom. (a) Assuming that transcription starts with the first $\mathrm{T}$ in the template strand, and continues to the end, what would be the sequence of the mRNA derived from this fragment?
(b) Find the initiation codon in this mRNA. (c) Would there be an effect on translation of changing the first $\mathrm{G}$ in the template strand to a $\mathrm{C}$ ? If so, what effect?
(d) Would there be an effect on translation of changing the second $T$ in the template strand to a G? If so, what effect? (e) Would there be an effect on translation of changing the last $\mathrm{T}$ in the template strand to a C? If so, what effect?