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Molecular Biology

Robert F. Weaver

Chapter 3

An Introduction to Gene Function - all with Video Answers

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Chapter Questions

01:36

Problem 1

Draw the general structure of an amino acid.

Mariana Roldan
Mariana Roldan
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03:50

Problem 1

Here is the sequence of a portion of a bacterial gene:
2'GTATCGTATGCATGCATCGTGAC3'
3'CATAGCATACGTACGTAGCACTG
The template strand is on the bottom. (a) Assuming that transcription starts with the first $\mathrm{T}$ in the template strand, and continues to the end, what would be the sequence of the mRNA derived from this fragment?
(b) Find the initiation codon in this mRNA. (c) Would there be an effect on translation of changing the first $\mathrm{G}$ in the template strand to a $\mathrm{C}$ ? If so, what effect?
(d) Would there be an effect on translation of changing the second $T$ in the template strand to a G? If so, what effect? (e) Would there be an effect on translation of changing the last $\mathrm{T}$ in the template strand to a C? If so, what effect?

Shiksha Dutta
Shiksha Dutta
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00:23

Problem 2

Draw the structure of a peptide bond.

Niamat Khuda
Niamat Khuda
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05:23

Problem 2

You are performing genetic experiments on Neurospora crassa, similar to the ones Beadle and Tatum did. You isolate one pantothenateless mutant that cannot synthesize pantothenate unless you supply it with pantoate. What step in the pantothenate pathway is blocked?

Amy Jackson
Amy Jackson
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07:01

Problem 3

Use a rough diagram to compare the structures of a protein $\alpha$-helix and an antiparallel $\beta$-sheet. For simplicity, show only the backbone atoms of the protein.

Anupa Sharad Medhekar
Anupa Sharad Medhekar
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02:26

Problem 4

What do we mean by primary, secondary, tertiary, and quaternary structures of proteins?

Asma Venkitta
Asma Venkitta
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06:17

Problem 5

What was Garrod's insight into the relationship between genes and proteins, based on the disease alcaptonuria?

Cody Delk
Cody Delk
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03:53

Problem 6

Describe Beadle and Tatum's experimental approach to demonstrating the relationship between genes and proteins.

Dennis Howard
Dennis Howard
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00:45

Problem 7

What are the two main steps in gene expression?

Joanna Quigley
Joanna Quigley
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Problem 8

Describe and give the results of the experiment of Jacob and colleagues that demonstrated the existence of mRNA.

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01:17

Problem 9

What are the three steps in transcription? With a diagram, illustrate each one.

Danielle Ashley
Danielle Ashley
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05:43

Problem 10

What ribosomal RNAs are present in E. coli ribosomes? To which ribosomal subunit does each rRNA belong?

Rabeya Zahid
Rabeya Zahid
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02:06

Problem 11

Draw a diagram of the cloverleaf structure of a tRNA. Point out the site to which the amino acid attaches and the site of the anticodon.

Shiksha Dutta
Shiksha Dutta
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01:32

Problem 12

How does a tRNA serve as an adapter between the 3-bp codons in mRNA and the amino acids in protein?

Andrei Demkov
Andrei Demkov
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01:46

Problem 13

Explain how a single base change in a gene could lead to premature termination of translation of the mRNA from that gene.

Shazia Naz
Shazia Naz
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04:06

Problem 14

Explain how a single base deletion in the middle of a gene would change the reading frame of that gene.

Amruta Pandit
Amruta Pandit
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01:40

Problem 15

Explain how a single base change in a gene can lead to a single amino acid change in that gene's polypeptide product. Illustrate with an example.

Aditya Sood
Aditya Sood
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