• Home
  • Textbooks
  • Biotechnology
  • Genomics and Gene Expression

Biotechnology

David P. Clark Nanette J. Pazdernik

Chapter 8

Genomics and Gene Expression - all with Video Answers

Educators


Chapter Questions

01:28

Problem 1

Which of the following is utilized in genomic research?
a. microsatellite polymorphism
b. restriction fragment length polymorphism
c. single nucleotide polymorphism
d. variable number tandem repeat
e. all of the above

Joanna Quigley
Joanna Quigley
Numerade Educator
00:19

Problem 2

What is contig mapping?
a. Determination of regions that overlap from one clone to the next in a library
b. The distance in base pairs between two markers
c. The use of landmarks in the genes to put together sequencing data
d. The relative order of specific markers in a genome
e. The mapping that determines if a library sequence is from one continuous gene or two gene segments cloned into one vector

Sam Limsuwannarot
Sam Limsuwannarot
Numerade Educator
01:43

Problem 3

Which method was used to sequence the human genome?
a. cytogenetic mapping
b. shotgun sequencing
c. chromosome walking
d. radiation hybrid mapping
e. All of the above were used in combination to complete the project.

Courtney Burson
Courtney Burson
Numerade Educator
01:26

Problem 4

Which organism has the most genes?
a. $H$. sapiens
b. D. melanogaster
c. O. sativa
d. P. trichocarpa
e. A. thaliana

Anitha Mary
Anitha Mary
Numerade Educator
01:20

Problem 5

What is a gene?
a. a segment of DNA that encodes a protein
b. a segment of DNA that encodes non-translated RNA
c. sequences of DNA that are not transcribed
d. a segment of DNA that is transcribed
e. all of the above

Christina Sorrentino
Christina Sorrentino
Numerade Educator
01:18

Problem 6

Which of the following is considered a field of study related to bioinformatics?
a. proteomics
b. computational biology
c. genomics
d. cheminformatics
e. all of the above

Christina Sorrentino
Christina Sorrentino
Numerade Educator
00:48

Problem 7

How is data mining useful to biotechnology research?
a. It allows researchers to determine sequence similarity, which usually translates into functional similarity.
b. Data mining allows researchers to use computers to study, sort, and compile the vast amounts of raw data generated through bioinformatics.
c. Data mining is the act of gathering the raw data from research projects such as sequencing into one central location.
d. Data mining usually provides too much information, which only slows down the research project and is therefore not very useful.
e. None of the above

Amrita Narasimhan
Amrita Narasimhan
Numerade Educator
01:39

Problem 8

Which type of mutation is the most common?
a. insertion of one or more bases
b. depletion of one of more bases
c. base substitutions
d. inversion of DNA segments
e. duplications of DNA segments

Qudsiya Anis
Qudsiya Anis
Numerade Educator
02:02

Problem 9

Which of the following statements about mutations is not true?
a. Mutations occur in all organisms at the same rate.
b. DNA polymerase never produces mutations during replication because of the proofreading ability of this enzyme.
c. Mutations often occur at methylated cytosine residues.
d. Mutations such as duplications or deletions occur due to repetitive sequences causing strand slippage.
e. When comparing mutation rates to coding capacity, mutation rates are usually the same for most organisms, which suggests a mechanism to control the rate.

Kathleen Jerome
Kathleen Jerome
Numerade Educator
01:02

Problem 10

Which one of the following is often used to establish family trees for organisms because it is present in all organisms and does not accumulate mutations quickly?
a. rRNA
b. fibrinopeptides
c. hemoglobin
d. chloroplasts
e. mitochondrial DNA

Jonathan Temple
Jonathan Temple
Numerade Educator
01:32

Problem 11

Which of the following statements about DNA microarrays is not correct?
a. Fluorescently labeled mRNA from the organism hybridizes to the DNA on the glass slide.
b. DNA microarrays contain thousands of DNA segments on a support, such as a glass slide.
c. Hybridization to a DNA microarray can only occur once.
d. The amount of fluorescence correlates with the amount of mRNA in the sample.
e. The data obtained from DNA microarrays represents a global view of gene expression, even under particular growth conditions.

Jonathan Temple
Jonathan Temple
Numerade Educator
02:10

Problem 12

What is the term used to describe the process of synthesizing oligonucleotides directly on the glass slide?
a. photosynthesis
b. photolithography
c. light-activated oligosynthesis
d. on-chip oligosynthesis
e. protected oligosynthesis

Shahina -
Shahina -
Numerade Educator
01:16

Problem 13

Which of the following statements highlights the issues surrounding oligonucleotide microarrays?
a. A duplex may not properly form if the mRNA probe has several mismatches compared to the oligonucleotide sequence.
b. The ability to hybridize to the oligonucleotides will be decreased if the probe is able to form a stem-loop structure.
c. The A:T content of the oligonucleotide may affect the stability of the duplex.
d. Depending on the size of the spacer, incoming probes may not be able to hybridize if the spacer is too small or the oligonucleotide may fold back on itself if the spacer is too long.
e. All of the above are issues surrounding oligonucleotide microarrays.

Joanna Quigley
Joanna Quigley
Numerade Educator
01:43

Problem 14

What can whole-genome arrays identify?
a. regions on the DNA that are methylated
b. transcription factor binding sites
c. various polymorphisms
d. repetitive elements
e. all of the above

Courtney Burson
Courtney Burson
Numerade Educator
00:47

Problem 15

Which one of the following fusion proteins does not require some kind of chemical substrate to observe activity?
a. luciferase
b. alkaline phosphatase
c. green fluorescent protein
d. $\beta$-galactosidase
e. all of the above

Dennis Howard
Dennis Howard
Numerade Educator

Problem 16

Why is RNA-Seq more advantageous than typical microarrays?
a. RNA-Seq does not need a probe.
b. Both coding and non-coding RNAs can be monitored with RNA-Seq.
c. Alternative splicing is detected by RNA-Seq.
d. Allele-specific expression can be monitored with RNA-Seq.
e. All of the above are advantages over typical microarrays.

Check back soon!
02:48

Problem 17

What technique could you use to simultaneously monitor host and pathogen RNA?
a. RNA-seq
b. microarrays
c. RT-PCR
d. gene fusions
e. ChIP

Sylvia Puglisi
Sylvia Puglisi
Numerade Educator
01:22

Problem 18

Epigenetics
a. is the total number of possible changes within a particular genome
b. refers to inherited changes in gene expression
c. is due to DNA modifications
d. is strictly limited to inherited proteins
e. results from DNA base changes

Christina Sorrentino
Christina Sorrentino
Numerade Educator
01:30

Problem 19

Which of the following is not an observed epigenetic change?
a. RNAi inheritance in nematodes and plants.
b. Inheritance of yeast prions, as observed through conformation changes in regulatory proteins.
c. The effects of DNA methylation and histone modification on the gene expression within a single cell.
d. Regulation of phase variation in bacteria due to DNA methylation patterns.
e. Persistance of siRNAs during cell division.

Christina Sorrentino
Christina Sorrentino
Numerade Educator
01:21

Problem 20

When a silenced allele is inherited from one parent, this is called
a. chromosome inactivation
b. imprinting
c. development and differentiation
d. environmental influence
e. maintenance of genome integrity

Christina Sorrentino
Christina Sorrentino
Numerade Educator