What technique could you use to simultaneously monitor host and pathogen RNA? a. RNA-seq b. micr

What technique could you use to simultaneously monitor host...

Key Concepts

Dual RNA-seq

Dual RNA-seq is a specialized application of RNA sequencing that simultaneously captures the transcriptomes of two interacting organisms, such as a host and a pathogen. This approach enables researchers to study the complex interactions and coordinated gene expression changes in both organisms during infection or other biological processes.

RNA-seq

RNA-seq is a high-throughput sequencing technique that analyzes the transcriptome by converting RNA into cDNA and sequencing it to provide a comprehensive snapshot of gene expression. This method can capture a wide range of RNA species, including low-abundance transcripts and novel RNAs, making it highly effective for detailed gene expression profiling.

Transcript

00:01 In this problem, we're asked about techniques that allow us to create a dna library.

00:06 And a dna library is just a collection of all of the genes in an organism.

00:12 So every single gene in an organism's genome.

00:15 And it's most often used for a bacterial genome or something else with a relatively small genome because of how it is constructed.

00:24 What techniques can we use to maybe get a dna library? one suggests it might be pcr or polymerase chain reaction, but pcr is more often used to amplify or increase the copies of a specific small piece of dna.

00:41 We also see the word sequencing.

00:44 Sequencing means finding the sequence of g's c's a &ts or nucleotides that are in a dna fragment.

00:51 That is something that we often do with genomes and bacterial genomes, but that's not creating a dna library.

00:59 Our next idea to consider is a microarray or a dna microarray, which is a really cool idea.

01:06 It's basically a collection of many different little dna spots on an array of wells that measures the expressions of large number of genes simultaneously.

01:19 It's a cool technique, but it's not the one that's going to be most applicable here.

01:25 Here we're going to want to use two main techniques in order to create and use a dna.

01:29 Library, cloning and colony blotting.

01:33 So when we want to create a dna library, our first mission is to take the organism whose genome we want to make a library of, usually a bacterium, and cut up all of its dna into small fragments.

01:45 Those fragments we're going to clone and insert into plasmids that go into well -characterized organisms like our e...

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