The original DNA sequencing procedure invented by Fred Sanger used a DNA template (the molecule whose sequence you wish to determine), a radioactively labeled oligonucleotide primer, DNA polymerase, deoxyribonucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), and dideoxyribonucleotide triphosphates (ddATP, ddCTP, ddGTP, ddTTP).
a) Explain why ddATP, ddCTP, ddGTP, and ddTTP were included in the sequencing procedure.
b) What will happen if the researcher mistakenly adds ten times less dideoxyribonucleotide triphosphates to the Sanger sequencing reaction?