00:01
For this question, there are many ways to detect a gene defect, but there is really only one good and approved way to correct it in most cases.
00:11
So gene defect is going to consist of some sort of mutation or error inside of a gene, causing it to produce a faulty protein.
00:23
So first, we have to go about detecting this.
00:26
In some cases, you might use an electrophoresis, which is good for detecting.
00:34
The size of certain dna strands.
00:39
If the gene defect were suspecting were the case of many duplications, or in the deletion, or in an area where we're able to detect this change in the dna structure through the use of restriction enzymes, these could all change the length of the dna products that we could detect running an electrophoresis.
01:10
And this is basically separating dna strands out by size, using an electrical current.
01:17
If those aren't appropriate for the gene defect we are suspecting, the other best way is to run a sequence, which is to have a machine grab a section of the dna that we suspect and to actually create complementary pairs using the nucleotides seen in dna.
01:41
From there, we use a sort of luminescent marker to be able to detect which nucleotide is in which position, and we can compare that to a database of the normal gene product...