00:01
We're talking about hydrolyases versus just lyases.
00:10
So hydrolases versus lyases.
00:19
Now, both of them can use water.
00:23
Liasis don't always use water.
00:25
Hydrolases do.
00:26
Hydrolases catalyze hydrolysis.
00:31
They catalyze hydrolysis, which we see all the time show up in all sorts of reactions in biochemistry.
00:43
And just as kind of a quick reminder, a classic hydrolysis would be having an ester.
00:51
We're just going to kind of make something up here.
00:57
And then we'll say he keeps going for a while.
01:04
All right.
01:06
Hydrolysis would be breaking the bond between the ester and the carbon chain.
01:13
So that would be right here.
01:16
And the way that it's broken and it's split into two pieces is by adding a water molecule where one side will end up with an additional hydrogen tecton.
01:26
The other side will end up with a hydroxide because if you break a water apart, you get a hydrogen and a hydroxide.
01:35
So then this creates, this bond goes away.
01:39
Now we've got two molecules.
01:42
All right.
01:43
This oxygen, it'll be at a weird angle just because of it.
01:45
The way we're trying to draw here.
01:46
That now has a hydrogen on it, and this one will now have a hydroxide.
01:53
So we broke it into two different molecules now.
01:56
Okay, that's your classic hydrolysis.
02:00
Lieses can use water.
02:01
They don't have to...