00:01
Okay, so the first question that we want to think about is, what is sds page? okay.
00:07
So sds is sodium doadessal sulfate.
00:14
So we have a sodium ion doadessel, simply a long carbon chain.
00:24
And at the head of that chain is a sulfate group, and the negative charge of the oxygens in that sulfate group are still.
00:33
Stabilized by sodium.
00:34
Okay.
00:36
So sds, sodium de -desolpate is the detergent that is going to be used to break down the bonds that are holding your protein together and to hopefully get them down to the monomeric state.
00:51
Okay.
00:52
That's typically what we want to look at when we do sds page.
00:55
However, there are forms of sds page that look at the native protein, so with all of its interactions intact.
01:02
The p .a.
01:04
G is polyacrylamide gel, which is simply what your gel is composed of.
01:12
And e stands for electrophoresis.
01:15
Okay.
01:17
When we do sds page, basically on our gel, we have this marker, and the marker kind of denotes the rough estimate of size or of the molecular weight of your protein...