Question
Compare and contrast the $\mathrm{S} 1$ mapping and primer extension methods for mapping the $5^{\prime}$-end of an mRNA. Which of these methods can be used to map the $3^{\prime}$-end of an mRNA. Why would the other method not work?
Step 1
The process involves hybridizing a single-stranded, labeled DNA probe to an RNA sample. The probe is designed to be complementary to a region just downstream of the suspected start of the mRNA. After hybridization, the RNA-DNA hybrid is treated with S1 nuclease, Show more…
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