Question

Here is a fragment of DNA that has been cut at certain points and will be run through a gel. In which order will the DNA fragments run, beginning at the wells? (A) $1,2,3,4,5$ (B) $5,4,3,2,1$ (C) $5,2,4,1,3$ (D) $5,2,3,1,4$ (E) $3,1,4,2,5$

Name: Problem 95, Chapter 6: SAT Biology E/M
Uploaded: 2020-10-20T01:55:43-08:00
Duration: 56 s
Channel: Joanna Quigley
Description: Here is a fragment of DNA that has been cut at certain points and will be run through a gel. In which order will the DNA fragments run, beginning at the wells? (A) 1,2,3,4,5 (B) 5,4,3,2,1 (C) 5,2,4,1,3 (D) 5,2,3,1,4 (E) 3,1,4,2,5

   Here is a fragment of DNA that has been cut at certain points and will be run through a gel. In which order will the DNA fragments run, beginning at the wells?
(A) $1,2,3,4,5$
(B) $5,4,3,2,1$
(C) $5,2,4,1,3$
(D) $5,2,3,1,4$
(E) $3,1,4,2,5$

SAT Biology E/M

Deborah Goldberg 5th Edition

Chapter 6, Problem 95

Instant Answer

Solved by Expert Joanna Quigley

10/20/2020

Step 1

This is because DNA is negatively charged, so the smaller fragments will move faster through the gel. Show more…

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Here is a fragment of DNA that has been cut at certain points and will be run through a gel. In which order will the DNA fragments run, beginning at the wells? (A) $1,2,3,4,5$ (B) $5,4,3,2,1$ (C) $5,2,4,1,3$ (D) $5,2,3,1,4$ (E) $3,1,4,2,5$

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Key Concepts

Gel Electrophoresis

Gel electrophoresis is a laboratory method used to separate DNA fragments based on their size by applying an electric field to a matrix (gel). DNA molecules, which are negatively charged, move through the gel toward the positive electrode, with smaller fragments moving faster and farther than larger ones.

DNA Fragment Separation Based on Size

The principle of DNA fragment separation relies on molecular size; smaller fragments traverse the gel more easily because they experience less resistance from the gel matrix. This results in an ordered distribution of fragments where the size differences can be inferred from the distance each fragment travels from the wells.

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