Question
How would diisopropylphosphofluoridate (DIPF; Section $11-5 \mathrm{A}$ ) affect the apparent $K_{M}$ and $V_{\max }$ of a sample of chymotrypsin?
Step 1
It binds to the enzyme chymotrypsin and permanently inactivates it by forming a covalent bond with a specific serine residue in the active site of the enzyme. Show more…
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