Question

Peas (Pisum sativum) are diploid and $2 n=14 .$ In Neurospora, the haploid fungus, $n=7$. If you were to isolate genomic DNA from both species and use electrophoresis to separate DNA molecules by size, how many distinct DNA bands would be visible in each species?

Name: Problem 15, Chapter 2: Introduction to Genetic Analysis
Uploaded: 2023-05-29T23:33:41-08:00
Duration: 1 min 56 s
Channel: Melanie Mew
Description: Peas (Pisum sativum) are diploid and 2 n=14 . In Neurospora, the haploid fungus, n=7. If you were to isolate genomic DNA from both species and use electrophoresis to separate DNA molecules by size, how many distinct DNA bands would be visible in each species?

   Peas (Pisum sativum) are diploid and $2 n=14 .$ In Neurospora, the haploid fungus, $n=7$. If you were to isolate genomic DNA from both species and use electrophoresis to separate DNA molecules by size, how many distinct DNA bands would be visible in each species?

Introduction to Genetic Analysis

Anthony J. F.… 11th Edition

Chapter 2, Problem 15

Instant Answer

Solved by Expert Melanie Mew

05/29/2023

Step 1

Peas (Pisum sativum) are diploid organisms with a total of 14 chromosomes (2n=14). This means that they have two sets of seven different chromosomes. On the other hand, Neurospora is a haploid fungus with seven chromosomes (n=7). This means that it has one set of Show more…

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Peas (Pisum sativum) are diploid and $2 n=14 .$ In Neurospora, the haploid fungus, $n=7$. If you were to isolate genomic DNA from both species and use electrophoresis to separate DNA molecules by size, how many distinct DNA bands would be visible in each species?

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Key Concepts

Chromosome Number and Homolog Pairing

Organisms have a specific number of chromosomes, but in diploid species the chromosomes come in homologous pairs that are nearly identical in size and structure. As a result, when genomic DNA is separated by electrophoresis, the two copies of each chromosome typically co-migrate, meaning that only one band is visible for each distinct chromosome type rather than one for every physical chromosome present.

Electrophoretic Separation of Genomic DNA

Techniques like pulsed?field gel electrophoresis allow the separation of very large DNA molecules based solely on size. In this method, chromosomes of distinct sizes appear as separate bands; however, if homologous chromosomes are similar in size they will overlap, leading to a number of visible bands that corresponds to the number of unique chromosome sizes rather than the total chromosome count.

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