Question

The promoter of an operon is the site to which $\mathrm{RNA}$ polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site to which RNA polymerase cannot bind. Would you expect mutations in the promoter that prevent binding of RNA polymerase to act in trans on another copy of the operon on a plasmid in the cell, or only in cis on the copy immediately adjacent to the mutated site?

Name: Problem 6, Chapter 16: Genetics: From Genes to Genomes
Uploaded: 2023-01-13T19:27:26-08:00
Channel: Farhan Anwar

   The promoter of an operon is the site to which $\mathrm{RNA}$ polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site to which RNA polymerase cannot bind. Would you expect mutations in the promoter that prevent binding of RNA polymerase to act in trans on another copy of the operon on a plasmid in the cell, or only in cis on the copy immediately adjacent to the mutated site?

Genetics: From Genes to Genomes

Leland Hartwell,… 6th Edition

Chapter 16, Problem 6

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Solved by Expert Farhan Anwar

01/13/2023

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The promoter is the region of DNA that initiates transcription of a particular gene. It is the site where RNA polymerase binds to start the transcription process. Show more…

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The promoter of an operon is the site to which $\mathrm{RNA}$ polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site to which RNA polymerase cannot bind. Would you expect mutations in the promoter that prevent binding of RNA polymerase to act in trans on another copy of the operon on a plasmid in the cell, or only in cis on the copy immediately adjacent to the mutated site?

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Key Concepts

Cis-acting Elements

Cis-acting elements are regions of DNA that regulate the expression of genes located on the same molecule of DNA. Changes in these elements, such as mutations in a promoter, affect only the gene adjacent to or near the mutation rather than influencing other copies of the gene elsewhere in the cell.

Promoter Region

The promoter is a key DNA sequence that defines where transcription by RNA polymerase begins. Mutations in the promoter typically disrupt the local binding of RNA polymerase, thereby affecting transcription initiation only for the adjacent operon and not for other copies that possess an intact promoter.

Trans-acting Factors

Trans-acting factors are usually proteins or other molecules that can diffuse within the cell to influence the expression of target genes regardless of their chromosomal location. In contrast to cis mutations, defects in trans-acting components can affect multiple copies of a gene or operon throughout the cell.

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