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What is the subunit structure of elF4F? Molecular masses are not required.
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eIF4F is a eukaryotic initiation factor involved in the initiation of translation. Show more…
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Subunit Composition of a Protein A protein has a molecular mass of 400 kDa when measured by gel filtration. When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of 180, 160, and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol, three bands are again formed, this time with molecular masses of 160, 90, and 60 kDa. Determine the subunit composition of the protein.
Quaternary structure. A protein was purified to homogeneity. Determination of the mass by gel-filtration chromatography yields 60 kDa. Chromatography in the presence of $6 \mathrm{M}$ urea yields a $30-\mathrm{kDa}$ species. When the chromatography is repeated in the presence of $6 \mathrm{M}$ urea and 10 mM $\beta$ -mercaptoethanol, a single molecular species of 15 kDa results. Describe the structure of the molecule.
A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), the protein gives three bands with molecular masses of $180,160,$ and 60 kDa. When electrophoresis is carried out in the presence of SDS and dithiothreitol, three bands are again formed, this time with molecular masses of 160,90 , and 60 kDa. Determine the subunit composition of the protein.
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