Which of the following is needed both in using bacteria to...

Name: Problem 8, Chapter 18: Biology: The Dynamic Science
Uploaded: 2021-07-29T23:15:25-08:00
Duration: 1 min 28 s
Channel: Joanna Quigley
Description: Which of the following is needed both in using bacteria to produce proteins and in genetic engineering of human cells? a. DNA fingerprinting based on microsatellite sequences b. insertion of a transgene into an expression vector c. restriction fragment length polymorphism (RFLP) d. screening of a cDNA library by DNA hybridization e. antibiotic resistance

Which of the following is needed both in using bacteria to produce proteins and in genetic engineering of human cells? a. DNA fingerprinting based on microsatellite sequences b. insertion of a transgene into an expression vector c. restriction fragment length polymorphism (RFLP) d. screening of a cDNA library by DNA hybridization e. antibiotic resistance

Which of the following is needed both in using bacteria to produce proteins and in genetic engineering of human cells?
a. DNA fingerprinting based on microsatellite sequences
b. insertion of a transgene into an expression vector
c. restriction fragment length polymorphism (RFLP)
d. screening of a cDNA library by DNA hybridization
e. antibiotic resistance

Labs

Want to see this concept in action?

NEW

Explore this concept interactively to see how it behaves as you change inputs.

View Labs

Key Concepts

Recombinant DNA Technology

This is the overall method of combining DNA from different sources to create new genetic combinations that are useful in biotechnology. It encompasses techniques used to transfer and express genes in diverse host organisms, whether bacteria for protein production or human cells for therapeutic or research purposes.

Transgene Insertion

Transgene insertion involves incorporating a gene of interest into a host’s genome or a cloning vector. This step is critical in both bacterial protein production and genetic engineering of human cells, as it enables the expression of the desired protein or trait by introducing the new genetic material into the host.

Expression Vector

An expression vector is a specially designed plasmid or virus that contains regulatory sequences required for gene expression. It serves as the vehicle for transgene insertion, ensuring that the introduced gene is efficiently transcribed and translated. This is a fundamental tool in both microbial production of proteins and the genetic modifications of human cells.

Transcript

Please give Ace some feedback