a. Into tube 1, pipette 4.5 mL of diluent.
b. Into tubes 2-10, pipette 0.5 mL of diluent.
c. Pipette 0.5 mL patient serum into tube 1 and mix thoroughly.
d. Transfer 0.5 mL of the contents of tube 1 into tube 2 and mix well.
e. Transfer 0.5 mL of tube 2 into tube 3 and mix well. Continue this dilution for the remainder of the tubes.
f. Remove and discard 0.5 mL from tube 10 after it has been mixed.
g. Add .03 mL antigen suspension.
9. What is the dilution in tube 3?