00:01
Serum and you pipette 0 .5 ml into 4 .5 ml dilution and you repeat it 10 times.
00:05
So the question says that what is the dilution factor in tube 1? 0 .5 ml to begin with and then the final volume is 0 .5 plus 4 .5.
00:26
0 .5 ml divided by 5 ml.
00:32
So this is a 1 to 10 dilution.
00:39
So you can see that the volume increased 10 times, the concentration drops 10 times.
00:44
So for the first question, the dilution factor is 10.
00:57
The next question, what is the dilution factor in tube 3? so you repeat this process twice.
01:04
So you have 1 to 10 first dilution and second tube 1 to 10.
01:11
Again, this is a single dilution and then in tube number 3 you repeat it one more time.
01:18
So you can see each time you dilute, you multiply by dilution factor.
01:21
So it's a 1 to 1000 dilution in tube number 3...