1) For this first prompt, let’s think about enzyme activity. A graph shows F-1,6-BPase activity in the presence or absence of F-2,6-BP (in presence, line is lower, in absence, line is higher). Based on this graph, does F-2,6-BP activate or inhibit F-1,6-BPase activity?
2) Hormonal signaling by insulin or glucagon influences F-2,6-BP levels, which in turn regulates metabolic flux (flow) through glycolysis and gluconeogenesis in liver cells.
a. Indicate what happens to F-2,6-BP levels when glucagon is present and what this change in F-2,6-BP does to the rate of glycolysis and gluconeogenesis.
b. In the context of metabolism, explain why it makes sense that glucagon signaling has the effect on glycolysis AND gluconeogenesis you mentioned in prompt ‘a’.