IV. THE EFFECT OF SUBSTRATE CONCENTRATION ON THE REACTION RATE (SECOND LAB PERIOD) In this experiment, the starch (substrate) concentration will be increasing while the amount of amylase (enzyme) remains constant. To calculate the rate of each reaction you will determine how much product was formed during a 10-minute reaction period. Hypothesis: Prediction: If the hypothesis is correct, then the outcome of this experiment should be that Procedure (This Is a Group Activity) 1. Describe how you will prepare your reaction tubes and run your experiment.
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I will mix the substrate (starch) and the enzyme (amylase) in a ratio that I know will create a reaction. Show more…
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Effect of Enzyme Concentration on Reaction Rate Discussion 1. Explain your results in terms of your hypothesis. Describe the shape of the reaction rate curve as enzyme concentration increases. What factors are responsible for the shape of this curve? (3 points) According to the graph the curve represent the 2. Speculate about the shape of a curve measuring reaction rate if you had increased the concentration of enzyme, but held the concentration of substrate constant. (3 points)
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