1. 0.1 mL of a protein solution of concentration of 8 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 3 mL of this solution was then mixed with 27 mL of water. What is the concentration of the diluted protein solution? (a) 0.2 mg/mL (b) 0.02 ?g/mL (c) 0.02 mg/mL (d) 0.4 mg/mL Answer: Space to show your workings:
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1 mL of 8 mg/mL protein solution to a total volume of 4.0 mL with water. Initial concentration (C1) = 8 mg/mL Initial volume (V1) = 0.1 mL Final concentration (C2) = ? Final volume (V2) = 4.0 mL Using the dilution formula: C1 * V1 = C2 * V2 8 mg/mL * 0.1 mL = C2 Show more…
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