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Questions: 1. You weigh 1.0 gram of protein and dissolve it in 1.0 mL of water to make a stock solution with a concentration of 1 gram / mL. 1a. (3pts) Using your 1g/mL stock solution (Hint: C1), you wish to make 100mL of a 10 mg/mL solution. (Hint: V2 and C2) How do you make this working solution? What is the DF for this dilution? (Hint: Review how to calculate DF in lab manual.) 100mL of 10mg/mL from 1g/ml stock | Volume Stock 1mL | Final Volume 100mL | D.F. 100 1b. (6pts) You also wish to make 10mL of a 0.01 mg/mL solution. (Hint: NEW V2 and C2). Calculate the two different ways to make this solution. 10mL of 0.01mg/mL from 1g/ml stock | Volume Stock 1x10^-4mL | Final Volume 10mL | D.F. 10^5 10mL of 0.01mg/mL from 10mg/ml stock | Volume Stock 0.01mL | Final Volume 10mL | D.F. 100 1c. (1pt) Which solution is a better choice to make the 0.01 mg/mL? In the second dilution is a better choice to make the 0.01mg/mL because there the volume of stock needed to make the final volume is greater than the other. 2. (2pts) Describe the correlation between protein concentration and absorbance in your data. TYPE ANSWER: 3. (2pts) Why do we need to make a standard curve for this experiment? TYPE ANSWER:

          Questions:
1. You weigh 1.0 gram of protein and dissolve it in 1.0 mL of water to make a stock
solution with a concentration of 1 gram / mL.

1a. (3pts) Using your 1g/mL stock solution (Hint: C1), you wish to make 100mL of a 10
mg/mL solution. (Hint: V2 and C2) How do you make this working solution?
What is the DF for this dilution? (Hint: Review how to calculate DF in lab manual.)

100mL of 10mg/mL from 1g/ml stock | Volume Stock 1mL | Final Volume 100mL | D.F. 100

1b. (6pts) You also wish to make 10mL of a 0.01 mg/mL solution. (Hint: NEW V2 and
C2). Calculate the two different ways to make this solution.

10mL of 0.01mg/mL from 1g/ml stock | Volume Stock 1x10^-4mL | Final Volume 10mL | D.F. 10^5
10mL of 0.01mg/mL from 10mg/ml stock | Volume Stock 0.01mL | Final Volume 10mL | D.F. 100

1c. (1pt) Which solution is a better choice to make the 0.01 mg/mL? In the second
dilution is a better choice to make the 0.01mg/mL because there the volume of stock
needed to make the final volume is greater than the other.

2. (2pts) Describe the correlation between protein concentration and absorbance in your data.
TYPE ANSWER:

3. (2pts) Why do we need to make a standard curve for this experiment?
TYPE ANSWER:

Questions:
1. You weigh 1.0 gram of protein and dissolve it in 1.0 mL of water to make a stock
solution with a concentration of 1 gram / mL.

1a. (3pts) Using your 1g/mL stock solution (Hint: C1), you wish to make 100mL of a 10
mg/mL solution. (Hint: V2 and C2) How do you make this working solution?
What is the DF for this dilution? (Hint: Review how to calculate DF in lab manual.)

100mL of 10mg/mL from 1g/ml stock | Volume Stock 1mL | Final Volume 100mL | D.F. 100

1b. (6pts) You also wish to make 10mL of a 0.01 mg/mL solution. (Hint: NEW V2 and
C2). Calculate the two different ways to make this solution.

10mL of 0.01mg/mL from 1g/ml stock | Volume Stock 1x10^-4mL | Final Volume 10mL | D.F. 10^5
10mL of 0.01mg/mL from 10mg/ml stock | Volume Stock 0.01mL | Final Volume 10mL | D.F. 100

1c. (1pt) Which solution is a better choice to make the 0.01 mg/mL? In the second
dilution is a better choice to make the 0.01mg/mL because there the volume of stock
needed to make the final volume is greater than the other.

2. (2pts) Describe the correlation between protein concentration and absorbance in your data.
TYPE ANSWER:

3. (2pts) Why do we need to make a standard curve for this experiment?
TYPE ANSWER:

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