A.) A eukaryotic DNA sequence that affects transcription at distant promoters is called a(n) _______.
a) Regulatory promoter
b) Enhancer
c) Core promoter
d) Activator
e) Mediator
B.) The yoda regulator protein binds to regulator sequences and stimulates transcription of the jedi operon. When the yoda protein binds to a small molecule, it is no longer capable of binding to DNA near jedi. The jedi operon is always expressed ("always on") if a mutation prevents the yoda protein from binding to the small molecule. Control of this operon is:
a) 1. Positive inducible
b) 2. Positive repressible
c) 3. Negative inducible
d) 4. Negative repressible
C.) Which of the following is an example of a spontaneous mutation?
a) The base analog, 5-bromouracil, causes a mutation from adenine to guanine.
b) The intercalating agent, acridine orange, causes the insertion of a guanine.
c) Ultraviolet radiation causes covalent bonds between a cytosine and an adjacent thymine on the same strand, that are then replaced with two thymines.
d) Wobble causes a guanine to be substituted for an adenine.
D.) A gene with the mRNA sequence: 5'-UAC UGG CCG UUA UGC GUU GAU AUA ACG-3' and produces a peptide with the amino acid sequence: Tyr Trp Pro Leu Cys Val Asp Ile Thr. A mutation in this gene creates a protein with the following sequence: Tyr Trp Pro Leu. What type of mutation is this? Select the best answer based on the information given.
a) 1. Nonsense mutation
b) 2. Missense mutation
c) 3. Silent mutation
d) 4. Suppressor mutation
e) 5. Gain of function mutation
f) 6. Reverse mutation
E.) A gene with the mRNA sequence: 5'-UAC UGG CCG UUA UGC GUU GAU AUA ACG-3' and produces a peptide with the amino acid sequence: Tyr Trp Pro Leu Cys Val Asp Ile Thr. A mutation in this gene creates a protein with the following sequence: Tyr Trp Pro Leu Tyr Val Asp Ile Thr. What type of mutation is this? Select the best answer.
a) 1. Deletion
b) 2. Silent mutation
c) 3. Somatic mutation
d) 4. Base substitution
e) 5. Insertion