A flask containing 100 mL and a batch bioreactor containing 1 L of the same nutrient broth were inoculated with 500 cells of a certain bacterial species. The flask was incubated on a mechanical shaker and aeration was introduced in the bioreactor at 37°C for 2 hrs. The final pH and cell density in the flask were found to be 5 and 10^5 cells/ml respectively, and in the bioreactor, the values were 7 and 10^12 cells/ml respectively. a) Calculate the doubling time of the bacteria in the flask and the bioreactor. Can you hypothesize why the flask culture attained a lower final cell density?
Added by Timothy O.
Step 1
- Flask: - Initial cell density: \( 500 \) cells in \( 100 \) mL, which is \( \frac{500}{100} = 5 \) cells/mL. - Final cell density: \( 10^5 \) cells/mL. - Bioreactor: - Initial cell density: \( 500 \) cells in \( 1 \) L, which is \( \frac{500}{1000} = 0.5 Show more…
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