1. Consider the peptide Lys-Glu-Trp and answer the following
questions:
a) Draw the structure of this peptide when all the ionizable
groups are fully protonated.
b)How many ionizable groups does his peptide have? Label the pKa
for each of the ionizable groups in the structure above.
c)Draw the appropriate titration curve for this peptide on graph
paper starting at pH 0 and ending at pH 14. Label the x-axis,
y-axis and pKa
d)Determine the overall net charge of this peptide at each full
equivalent point (including 0) where the ionizable group is 100%
deprotonated.
e) Determine the pI for this peptide.
f) Determine the average overall net charge of a mixture of this
peptide in a solution where pH=8.5