1. How many transformed E. coli cells were put on the +DNA/AMP/IPTG plate when it was inoculated on day 1 of this experiment? How do you know?
2. Look for satellite colonies, small beige colonies which form in the near vicinity of a colony of transformed cells. Explain why these colonies are beige (and not green or blue) and why they are present ONLY near transformed colonies.
3. How does the AmpR gene, and agar containing ampicillin, help us find the very rare transformed E. coli among the very abundant untransformed cells in the +DNA tube? Hint: Ampicillin is a selective ingredient in the agar plates containing.
4. We transform bacteria to make medically useful human proteins such as insulin and growth hormone. Explain how the experiment we did could be modified so that we could:
a) transform E. coli cells to manufacture (not green or blue proteins), but rather, human insulin protein molecules.
and
B) then produce huge quantities of human insulin protein molecules? (over 8 million people in the USA alone need insulin injections daily) Hint: there's an item in the transformation module that has relevant info.
ronigen
The same +DNA/AMP plat under UV light
-DNA
+DNA +Amp
-DNA
The +DNA/AMP/IPT(
regular room lighting
+DNA LAMD +IPTG
DNA AMp
-DNA tAMP
The same +DNA/AMP/IPTG plate, photographed under l lighting
+DNA +AMD +IPTG
DN