00:01
The question says here we are asked to write about ampicillin and also arabinose in the pglo experiment or transformation experiment.
00:16
Now another part of the question is asking us to write where the non -transformants can be seen growing in the plate or which plate will have the non -transformants growing.
00:31
Coming to the plasmid gelo.
00:35
This plasmid gelo, as you can see in the picture has three components.
00:45
The first region is the beta lactamase which will be providing the ampicillin resistance to the bacteria and second one is the gfp that is the green fluorescent protein which will act as a selectable marker and the third one is the arac gene.
01:08
So this this arac gene is the arabinose gene which will be helping in regulating the production of protein, that is the gfp.
01:20
Now coming to the ampicillin, the role of ampicillin is here to act as a selectable marker.
01:27
What is a selectable marker? selectable marker is the one which differentiates the transformance from the non -transformants.
01:35
We want transformants in the end, right? so this ampicillin will be helping us to identify that transformed cells...