1. Which of the following is the correct definition of synteny? a) The conservation of gene order within two genomes b) The conservation of gene function within two genomes c) The percentage of nucleotide sequence identity between two genomes d) The percentage of amino acid sequence identity between two genomes 2. Why is open reading frame scanning not applicable when searching for functional RNA genes? a. The codons for functional RNA molecules are not well understood b. Introns make the identification of functional RNAs more difficult c. Functional RNA molecules are not composed of codons d. The genetic code is not universal e. The rules for specifying the start and stop regions of functional RNA genes are not well understood 3. Intrastrand base pairing can interfere with which of the following methods in DNA sequencing? a. Chemical degradation method b. Pyrosequencing c. Chain termination method d. Clone Contig method 4. Chromosome walking is best described as: a) Aligning DNA sequences by computer, to generate contigs b) Generating a map along a chromosome in a step by step manner c) Identifying clones whose inserts overlap to generate a library of clones that cover a given segment of DNA d) Sequencing a genome on one clone at a time to ensure that no gaps are present at the end of the project 5. Chromosome walking is best described as: a) Aligning DNA sequences by computer, to generate contigs b) Generating a map along a chromosome in a step by step manner c) Identifying clones whose inserts overlap to generate a library of clones that cover a given segment of DNA d) Sequencing a genome on one clone at a time to ensure that no gaps are present at the end of the project 6. Which of the following is NOT true about Next Generation Sequencing? a) Next-generation sequencing includes pyrosequencing and Illumina sequencing b) Next-generation sequencing sequences smaller fragmented DNA than Sanger sequencing c) Next-generation sequencing visualizes the sequence by fluorescent d) Next-generation sequencing does not require DNA polymerase
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Synteny refers to the conservation of gene order within two genomes. So, the correct answer is: $\boxed{\text{a) The conservation of gene order within two genomes}}$ Show more…
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4-1) Positional cloning involves which of the following? a. Walking along a chromosome from a marker to a nearby gene. b. Assembling clone contigs for an entire genome. c. Identifying genes present in genomic sequences. d. Fingerprinting a chromosome or DNA fragment to provide a map for sequencing 4-2) What is the most difficult and time-consuming aspect of a shotgun sequencing project? a. The generation of the clone libraries. b. The sequencing of the clone libraries. c. The generation of contigs from the DNA sequences. d. The closure of sequence and physical gaps between the sequenced contigs. 4-3) Why were yeast artificial chromosomes (YACs) not used in the sequencing phase of the Human Genome Project? a. It was discovered that some YACs contained cloned DNA fragments from different parts of the genome. b. The YAC inserts were too large to yield a manageable clone library for sequencing. c. Yeast genomic DNA was found to be recombining with human DNA in the YACs. d. YACs were found to lose large segments of insert DNA over time.
Adi S.
QUESTION 18 Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid? A. DNA ligase sites. B. Restriction enzyme sites. C. Origin of replication. D. Lac operator. QUESTION 19 The correct order of DNA segments in an operon is: A. Operator, structural genes, promoter. B. Promoter, operator, structural genes. C. Operator, promoter, structural genes. D. Promoter, structural genes, operator. QUESTION 20 The DNA sequence of the expression plasmid adjacent to the ribosome binding site is: ---GGATCCATCTGCGAATTC---- ---CCTAGGTAGACGCTTAAG--- It contains a BamH1 site (G^GATCC) and an EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end? A. EcoR1, followed by EcoR1. B. BamH1, followed by BamH1. C. BamH1, followed by EcoR1. D. EcoR1, followed by BamH1. QUESTION 21 When producing a protein from a gene on DNA, the first process to occur must be A. mutation B. translation C. replication D. transcription QUESTION 22 In an expression plasmid, the origin of replication: A. allows the bacteria to duplicate the plasmid. B. allows for growth of plasmid-containing bacteria in the presence of antibiotics. C. is responsible for the replication of mRNA. D. Is the position where the base number begins at 1. QUESTION 23 Separation of the original DNA strands is due to the action of: A. polymerase B. helicase C. single-stranded binding protein D. RNA primers QUESTION 24 Which of the following terms refers to a region of a chromosome that has a specific function? A. Genotype B. DNA C. Gene D. Allele QUESTION 25 The tRNA with UAU as the anticodon would be attached to which amino acid? A. leucine B. tyrosine C. isoleucine D. serine
1. Which of the following statements is FALSE? a. Non-homologous end joining is one way that inversions and translocations occur. b. Repair of CRISPR/Cas9-induced double strand breaks in mitotically dividing cells can occur either by non-homologous end joining or by homologous recombination. c. The DNA damage checkpoint triggers arrest in G2 in response to a double strand break. d. Mutations in some genes required for DNA repair are associated with cancer. e. CRISPR/Cas9 is a DNA-protein complex that makes targeted double strand breaks. 2. Base analogs such as 5-bromouracil or 2-aminopurine are mutagenic because: a. They intercalate between adjacent base pairs, causing insertion or deletion of bases during DNA replication. b. They induce the formation of pyrimidine dimers. c. They can switch between alternate forms that have different base-pairing specificities. d. They inhibit the proofreading activity of DNA polymerase. e. These base analogs are not mutagenic. 3. A yeast geneticist isolates six independent recessive mutants that are auxotrophic for leucine. Each mutant is designated leu-1, leu-2, etc. She crosses each mutant by itself as well as each of the other five Leu- mutants and tests the resulting diploids for their ability to grow on medium lacking leucine. She obtains the results shown on Fig. IV (Handout) and above, where "-" equals failure to grow; "+" equals growth on medium lacking leucine. How many mutants are present in the complementation group (gene) that contains the leu-6 mutant? a. 0 b. 1 c. 2 d. 3 e. 4 4. Which of the following statements about DNA replication is FALSE? a. Leading strand DNA synthesis is continuous and moves in the direction of the replication fork. b. When DNA polymerase incorporates the wrong nucleotide during DNA replication, the mismatch is corrected by replacing the nucleotide on the template strand with a nucleotide complementary to the nucleotide on the newly replicated DNA. c. Lagging strand DNA synthesis occurs because DNA polymerase can only add nucleotides in the 5' to 3' direction. d. Eukaryotic chromosomes are replicated using multiple origins of replication along the same chromosome. e. In eukaryotic cells, origins of replication are licensed so that each chromosome replicates only once in each cell cycle.
Suman K.
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