Question

QUESTION 18 Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid? A. DNA ligase sites. B. Restriction enzyme sites. C. Origin of replication. D. Lac operator. QUESTION 19 The correct order of DNA segments in an operon is: A. Operator, structural genes, promoter. B. Promoter, operator, structural genes. C. Operator, promoter, structural genes. D. Promoter, structural genes, operator. QUESTION 20 The DNA sequence of the expression plasmid adjacent to the ribosome binding site is: ---GGATCCATCTGCGAATTC---- ---CCTAGGTAGACGCTTAAG--- It contains a BamH1 site (G^GATCC) and an EcoR1 site (G^AATTC). In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end? A. EcoR1, followed by EcoR1. B. BamH1, followed by BamH1. C. BamH1, followed by EcoR1. D. EcoR1, followed by BamH1. QUESTION 21 When producing a protein from a gene on DNA, the first process to occur must be A. mutation B. translation C. replication D. transcription QUESTION 22 In an expression plasmid, the origin of replication: A. allows the bacteria to duplicate the plasmid. B. allows for growth of plasmid-containing bacteria in the presence of antibiotics. C. is responsible for the replication of mRNA. D. Is the position where the base number begins at 1. QUESTION 23 Separation of the original DNA strands is due to the action of: A. polymerase B. helicase C. single-stranded binding protein D. RNA primers QUESTION 24 Which of the following terms refers to a region of a chromosome that has a specific function? A. Genotype B. DNA C. Gene D. Allele QUESTION 25 The tRNA with UAU as the anticodon would be attached to which amino acid? A. leucine B. tyrosine C. isoleucine D. serine

QUESTION 18
Which DNA sequences must a synthetic gene contain in order for the gene to be inserted into the expression plasmid?
A.
DNA ligase sites.
B.
Restriction enzyme sites.
C.
Origin of replication.
D.
Lac operator.

QUESTION 19
The correct order of DNA segments in an operon is:
A.
Operator, structural genes, promoter.
B.
Promoter, operator, structural genes.
C.
Operator, promoter, structural genes.
D.
Promoter, structural genes, operator.

QUESTION 20
The DNA sequence of the expression plasmid adjacent to the ribosome binding site is:
---GGATCCATCTGCGAATTC----
---CCTAGGTAGACGCTTAAG---
It contains a BamH1 site (G^GATCC) and an EcoR1 site (G^AATTC).
In order for the synthetic gene to be inserted between these two sites, it should be constructed to have which restriction sites at its beginning and at its end?
A.
EcoR1, followed by EcoR1.
B.
BamH1, followed by BamH1.
C.
BamH1, followed by EcoR1.
D.
EcoR1, followed by BamH1.

QUESTION 21
When producing a protein from a gene on DNA, the first process to occur must be
A.
mutation
B.
translation
C.
replication
D.
transcription

QUESTION 22
In an expression plasmid, the origin of replication:
A.
allows the bacteria to duplicate the plasmid.
B.
allows for growth of plasmid-containing bacteria in the presence of antibiotics.
C.
is responsible for the replication of mRNA.
D.
Is the position where the base number begins at 1.

QUESTION 23
Separation of the original DNA strands is due to the action of:
A.
polymerase
B.
helicase
C.
single-stranded binding protein
D.
RNA primers

QUESTION 24
Which of the following terms refers to a region of a chromosome that has a specific function?
A.
Genotype
B.
DNA
C.
Gene
D.
Allele

QUESTION 25
The tRNA with UAU as the anticodon would be attached to which amino acid?
A.
leucine
B.
tyrosine
C.
isoleucine
D.
serine

Added by Gabriella R.