00:01
Hello student here the question is regarding the true and false we have to identify whether the statement is true or false.
00:07
So first statement is the rna primer act as a starting point for the tac polymerase.
00:12
So we know the in case of the synthesis of the dna, the rna primer is needed for the addition of new nucleotide by using this tac polymerase.
00:23
So this is the true.
00:26
And if second one is the effect cut a dna in a plasmid origin of replication, then plasmid, then plasmid.
00:31
It failed to replicate and we know the plasmid is the extra chromosomal genetic material and for the replication it needs the origin of replication if there is any cut in between these then it cannot replicate so it is also true and the third one is the some restriction enzyme make jic -jack cut in double stranded dna and we know the restriction enzyme are the specific and they cut at the restriction side and the restriction enzyme can make either the sticky end or blunt end.
01:07
So these are not going to make any jigsack cut.
01:11
The sticky in cut is this kind of a structure.
01:16
So if there is cut is like this then this is the sticky end and blunt is suppose this is the whole dna and between these.
01:26
So these kind of blunt ink.
01:28
So there is no jigs jack cut.
01:31
So this is the false.
01:33
So 18 should be false and 19 a dna sequence will be cut using a particular restriction enzyme the longer the restriction side of the enzyme or the more fragment you would expect after digestion so if there is a longer side of the restriction site for the enzyme then there will be less fragment because if the site is more than the we have to cut into very less fragment.
02:02
So it is also false.
02:05
And if we look at the 20th, then the gel electrophiluses and this is the process where the charged particle moved under electric field and we know the dna is the negatively charged.
02:18
And here we can separate the dna on the basis of their size.
02:22
So these kind of structure of the gel and the larger dna molecule come closer to the well and the smaller one is the go far so it is the large size of the dna and this will be the small size of the dna so it based on the their size they can separate so this is the true and if we look at the this one then if only a smaller degraded sample available so pcr is used we know the pcr is a polymer chain reaction and in this this at the crime scene if we we get a very small amount of dna as a blood sample or any hair or follicles or we are getting, then we can amplify these dna by using this pcr.
03:11
So this is the true.
03:13
And this is a useful in case of crime or we can say the identification of the and this is the restriction enzyme break ionic point.
03:27
So we know the restriction enzyme suppose this is the dna sequence...