1. Which reaction besides lactose fermentation do HE, XLD and SS agars demonstrate?
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These are selective and differential media used primarily for the isolation and differentiation of enteric Gram-negative bacteria. Show more…
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Following applications? Used for which of the following: - ccpOIlA Telcr"$ Iron ap35, hccqusl Uydrogen Sulfide production Testing T0T Testing for Glucose fermentation Testing for Lactose fermentation Je4 of the natc Can utilization will produce color change positive reaction for Citrate ' From deep blue E Dosti From red to yellow Fron colorless to red From green to deep blue the following questions Answer True (A) False (H) collocation addition of Nitrate Nitrate broth 5t4 Fokn oition bacterial come Inan s reaction (or Nitrate This indicates; reagents Oxidation activity Pseudomonas Originated positive emt bcu [ ecinaly to Productive cltulied Streptococcus rQn alpha hemolytic classified "Streptococcus pyogenes FosAYe inutitol fermenting and coagulase Staphylococcus Oyek Tecuhc ccdus Rhn Idifferential medium js @ selective and Which of the followingE Mannitol Salt Agar MacConkey Agar Surhykroral Nieuun_ C; M Both All of the ktr The role of sodium chloride chloride, and FlenGl mannitol 7,59 E coli Salt _ Aen Contains mannitol medium positive bacteria To select for all gram negative bacteria From other than Staphylococci Ieuin 110 (SM 110) To Select for &ll worth Tof organisms Staphylococcus tnmibat TonL Mannitol Salt agar (MSA) is differentiate Gram negative for the growth of differential medium Selective Qota of the following Which bacteria? MacConkey Agar Hektoen Agar (XLD) = Agar Desoxycholate 5 C Xylose Lysine Medium (FTM) Fluid Thioglycollate =
Madhur L.
Nutrient agar is general purpose nutrient media used for cultivation of microb growth of wide range of non-fastidious organisms. Nutrient agar is popular be variety of types of bacteria and fungi and contains many nutrients needed for Due to it comparison of growth quality of various microorganisms can be don Q3. Proteus species and certain species of Salmonella produce a black precipit on Hektoen. Chemically what is occurring to cause the black precipitate to for ingredients in the media are being used by the bacteria to produce the black pre Q4. With respect to MacConkey agar, what would be the possible consequence a)replacing the lactose with glucose? Would you be able to distinguish EC from glucose instead? Explain. (Exercise 7 results will help here with this question). b)replacing the neutral red with phenol red (yellow when acidic; red or pink when Q5. For these questions refer to introductory pages of Ex. 5-20: a)What happens to blood agar when an alpha-hemolytic reaction occurs? b)What happens when a beta-hemolytic reaction occurs? c)What happens when a gamma-hemolytic reaction occurs?
Sana R.
76. A candle jar could be used for growing... (a) Gram negative organisms (b) facultatives (c) aero-tolerants (d) anaerobes (e) none of these is incorrect 77. How could you EASILY separate a facultative from an obligate anaerobe? (a) in an anaerobic jar (b) consider all of these methods (c) in a Carbon dioxide incubator (d) growth times (e) reproductive rate 78. You inoculated your Phenol Red Broth on Monday morning and incubated it overnight at Optimum Temperature. As you recover the tube from the incubator on Tuesday afternoon you observe that it has a pink fluid inside filling the Durham tube; the rest of the fluid is bright yellow but you forget to take it out. The next Monday you check it and it is pink again. What's going on? (a) record that carb as a Fast positive carb with negative pink gas (b) in your notes write answer c and reversion (c) record the media as a Fast positive without gas & with involution then, remove the marks and discard properly (d) record the media as a SLOW positive without gas then, remove the marks and properly discard the media (e) record it as negative for the carb and remove the marks and discard properly 79. Hydrolysis of the major protein in MILK detected by the _?_ test. (a) Litmus Milk (b) Skim Milk agar (c) Casein agar (d) Casease Test (e) none of these is incorrect 80. Which streak isolation method was demonstrated by your instructor as the MOST effective for student use and was recommended for your class? (a) 3-5 stripe quadrant streaking (b) backwards "S" or Z-streaking (c) the continuous streak (d) the "T" streak (e) the Radiant streak 81. Pink "fuzzing" away from the needle stab stab, or observation of "steps" of growth on a plate are often utilized to detect _?_ in unknown cultures. (a) oxygen production (b) flagella (c) indole (d) a cloudy Koser's and blue on a Simmon's test (e) Brownian motion 82. This test saves "puppies." (a) Ames (b) the test that demonstrates the diauxic growth (c) ONPG (d) answers a & c only (e) orange agar with hot pink growth 83. Which of the following is NOT a step in the mutant "Ninja" permease Test? (a) the test media "split" into an empty clean test tube (no top needed) (b) a 48 hr slant growth from a diauxic test media (c) about 2 ml of tap water (d) incubate or set O/N (e) all of these are correct 84. Enterobacter aerogenes may show a greenish "tinge" on EMB agar, what test would you select to prove that your unknown is really E. aerogenes and not a bacteria that indicates something dangerous but appearing similar? (a) PR lactose (b) Litmus milk (c) IMViC (d) a Citrate test (e) MR 85. Lactose & hydrogen sulfide positive fermenting bacteria growing on Hektoen agar is _?_. (a) burgundy or black/purple (b) black/pink(c) blackish metallic green (d) black/orange (e) clear/dark brown 86. BEFORE catalase breaks down Hydrogen peroxide to water and oxygen gas, the _?_ enzyme acts to break down "super" oxygen. (a) oxidase (b) dimutase (c) citritase (d) urease (e) none of these is correct 87. This bacteria would grow green or blue-green on green agar but wouldn't ever show "crystals" in the growth pattern. (a) Salmonella (b) Shigella (c) coliforms (d) E. coli (e) E. aerogenes 88. This bacteria would grow green or blue-green on green agar with dark "crystals" in the growth pattern. (a) Salmonella (b) Shigella (c) coliforms (d) E. coli (e) E. aerogenes 89. The reagent "Kovac's" is used in the _?_ test. (a) Nitrate (b) Indole (c) VP (d) MR (e) Tryptone agar 90. Your results indicate that your PR Lactose and Litmus Milk tests are pink. Your decision is.. (a) Dextrose is near neutral (b) Lactose is positive (c) the Milk protein test is positive (d) Casease is positive (e) ONPG is white
Adi S.
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