10. For your group project, you are interested in looking at...

10. For your group project, you are interested in looking at whether or not astaxanthin triggers an apoptotic response in HL-60 cells. For your experiment, you have read that the compound works best at 10 mM. Because you are using DMSO as a control and adding only 2 µL to a separate 2 mL plate, you want to add the same amount of astaxanthin. What concentration should you make your working solution of astaxanthin in order to make the 2 µL addition? After you find out your concentration, you find a trustworthy vial of astaxanthin in the lab, which is at a concentration of 70 M. How would you make 100 µL of your new desired concentration (using DMSO as a solvent)?

Transcript

00:01 So you have a group project, and you're interested in looking at whether or not estectarthing trigger and autosis response in the halociccicil.

00:10 So in your experiment, you've read that the compound works the best at 10mmol.

00:15 And because you are using dmso as in control, and you only add two micliter into a separate two -millimeter plate, you want to know what's the concentration of estexecisine would you make into a working solution in order to add the same amount of estaxidine into the 2 -millimeter plate.

00:36 So we can use this very useful formula, c1 times v1 equals c2 times v2.

00:45 C1 is the original or stock concentration of a solution.

00:49 V1 is the stock of volume that you want to take out.

00:52 C2 is the final concentration and v2 is the final volume.

00:57 Now, c1 is the concentration that we want to figure out of stethysticism.

01:04 And we know that we want to have a stock volume of 2 micliter because this is the volume of the m .s .o.

01:13 You want to add into the 2 milliliter plate.

01:17 C2, we know that the best concentration works for this compound is 10 millimolar.

01:22 So the final concentration is 10 millimolar.

01:25 Now the v2, the final volume, as we just mentioned, is 2 militer.

01:31 But since we want to be consistent with the unit, when i use mic liter there, and i want to make sure that it's also mic liter at the end.

01:40 So then since 1 militer equals 1 ,000 mic liter, 2 militer convert into 2 ,000 mic liter.

01:49 So now you can see both has micliter as a unit.

01:51 So we want to make sure the units are consistent on both sides.

01:55 Of the equation.

01:57 So now all we have to do is solve c1, the original concentration, the working solution.

02:02 C1 equals 10 times 2 ,000 divided by 2.

02:06 So the answer is 10 ,000 millimolar.

02:10 Now obviously we also know that 1 molar equals 1 ,000 millimolar.

02:16 So since this is a very big number, we want to convert millimolar to molar.

02:20 So 10 ,000 millimeter divided by 1 ,000 equals 10 ,000.

02:25 Molar.

02:26 So the working solution or the stock solution concentration of esthetazithin should be 10 millimolar to give a 2 -micliter and mix up into 2 -millimeter of final volume will give you a final concentration of 10 -millimeter.

02:45 Now let's move on to the second part of the question...

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