00:01
Hello.
00:02
So the question here is the question is asking for the three features of plasmid cloning vector, right? and that these three features will allow researchers to insert a piece of foreign dna and make copies of it in e.
00:22
Coli.
00:23
Also, they are asking for the purposes of these three features.
00:28
So first of all, let's see that what are plasmids? here these plasmids, these are actually a good cloning vectors, right? these are the cloning vectors.
00:48
These are very small in size and because they're very important characteristic is that they are autonomously or say self.
01:02
Replicating in the cell they are self replicating what double stranded dna molecule here we can write double standard dna molecule so here it is a piece of dna in other terms we can say that this is a piece of dna which is not the same as chromosomal dna.
01:28
So this is not as chromosomal dna, right? and its ability to replicate is independent of chromosomal dna.
01:45
Right.
01:46
So what we understand that this is a piece of dna here, but not chromosomal.
01:50
Now coming to the features as they are asked in the question about the three features.
01:55
So here the features.
01:56
The first one here is first feature is that origin of replication origin of replication can also be written as here ori right now this is what the purpose of this is what here this is the site they're asking for the purpose so this is the site where dna replication is initiated.
02:36
So here dna replication is initiated.
02:45
So this is the site.
02:49
Now here if we see the example of this can be plasmid mb1...