Question

15. (3) Which one of the following statements correctly describes the process of DNA sequencing by chain termination (the Sanger reaction)? a. the dideoxynucleotides lack 5'-OH groups and therefore can not serve as acceptors in nucleotide addition. b. in order to sequence DNA effectively, dideoxynucleotides must be present at a higher concentration than deoxynucleotides. c. the use of four unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run. d. the enzyme DNA polymerase is used to form a primer that binds to the template DNA. e. both B and C are correct.

          15. (3) Which one of the following statements correctly describes the process of DNA sequencing by chain termination (the Sanger reaction)?
a. the dideoxynucleotides lack 5'-OH groups and therefore can not serve as acceptors in nucleotide addition.
b. in order to sequence DNA effectively, dideoxynucleotides must be present at a higher concentration than deoxynucleotides.
c. the use of four unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run.
d. the enzyme DNA polymerase is used to form a primer that binds to the template DNA.
e. both B and C are correct.

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15. (3) Which one of the following statements correctly describes the process of DNA sequencing by chain termination (the Sanger reaction)?
a. the dideoxynucleotides lack 5'-OH groups and therefore can not serve as acceptors in nucleotide addition.
b. in order to sequence DNA effectively, dideoxynucleotides must be present at a higher concentration than deoxynucleotides.
c. the use of four unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run.
d. the enzyme DNA polymerase is used to form a primer that binds to the template DNA.
e. both B and C are correct.

Added by John D.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

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Solved by Expert Adi S

06/28/2023

Step 1

This results in the termination of DNA synthesis when a ddNTP is incorporated into the growing DNA strand. So, the first statement is incorrect as it mentions 5'-OH groups, which are not relevant in this context. To sequence DNA effectively, a mixture of Show more…

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Which one of the following statements correctly describes the process of DNA sequencing by chain termination (the Sanger reaction)? a. the dideoxynucleotides lack 5'-OH groups and therefore can not serve as acceptors in nucleotide addition. b. in order to sequence DNA effectively, dideoxynucleotides must be present at a higher concentration than deoxynucleotides. c. the use of four unique fluorescent labels attached to the dideoxynucleotides allows for a single reaction to be run. d. the enzyme DNA polymerase is used to form a primer that binds to the template DNA. e. both B and C are correct.

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