Please label the Images to review the process of polymerase chain reaction and how its products can be analyzed using gel electrophoresis. (+) Restriction fragments DNA Replication (-) Restriction endonucleases selectively cleaving sites of DNA Denaturation Priming Wells DNA Sample Strands separate. Heat to 94°C 50°C to 65°C Oligonucleotide primers attach at ends of strands to promote replication of amplicons. Amplicons Primer 72°C Heat-stable DNA polymerase synthesizes complementary strand. Polymerase Original strands Cycles 3, 4, ... repeat same steps. 1 2 3 4 5
Added by Anthony B.
Close
Step 1
Step 1: Identify the different components in the PCR diagram: - DNA sample - Denaturation - Annealing - Extension - New strands and original strands - Complete copies Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 61 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
17% of the factory's products are faulty. If you buy all of the factory's products, what is the probability that all of them are faulty? Give your answer as a decimal rounded to 2 decimal places.
Harmender Singh Y.
BIOTECHNOLOGY I POP BEAD CLONING 21. If the recombinant plasmid were cut with the following enzymes and combinations of enzymes, draw the way the fragments would appear on the finished gel after electrophoresis and staining. Use the map of the recombinant plasmid, rather than the pop beads, to determine the size of all the resulting fragments in each digestion. 1. Kb ladder 2. Pkpk 3. Pkyl 4. Ylyl 5. Pkpk + Pkyl 6. Pkpk + Ylyl 7. Pkyl + Ylyl 8. Uncut recombinant plasmid Sources: Clark, David P., and Lonnie D. Russell. Molecular Biology Made Simple and Fun 2nd Edition. Vienna, IL Cache River Press, 2000.
Madhur L.
$21-24$ Dot Products Find the indicated quantity, assuming that $\mathbf{u}=2 \mathbf{i}+\mathbf{j}, \mathbf{v}=\mathbf{i}-3 \mathbf{j},$ and $\mathbf{w}=3 \mathbf{i}+4 \mathbf{j}$ $$\mathbf{u} \cdot \mathbf{v}+\mathbf{u} \cdot \mathbf{w}$$
Vectors in Two and Three Dimensions
The Dot Product
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD