Question

BIOTECHNOLOGY I POP BEAD CLONING 21. If the recombinant plasmid were cut with the following enzymes and combinations of enzymes, draw the way the fragments would appear on the finished gel after electrophoresis and staining. Use the map of the recombinant plasmid, rather than the pop beads, to determine the size of all the resulting fragments in each digestion. 1. Kb ladder 2. Pkpk 3. Pkyl 4. Ylyl 5. Pkpk + Pkyl 6. Pkpk + Ylyl 7. Pkyl + Ylyl 8. Uncut recombinant plasmid 12 Kb 6 5 4 3 2 1.5 1.0 0.5 Sources: Clark, David P., and Lonnie D. Russell. Molecular Biology Made Simple and Fun 2nd Edition.. Vienna, IL Cache River Press, 2000.

          BIOTECHNOLOGY I
POP BEAD CLONING
21. If the recombinant plasmid were cut with the following enzymes and combinations of enzymes, draw the way the fragments would appear on the finished gel after electrophoresis and staining. Use the map of the recombinant plasmid, rather than the pop beads, to determine the size of all the resulting fragments in each digestion.

1. Kb ladder
2. Pkpk
3. Pkyl
4. Ylyl
5. Pkpk + Pkyl
6. Pkpk + Ylyl
7. Pkyl + Ylyl
8. Uncut recombinant plasmid

12 Kb
6
5
4
3
2
1.5
1.0
0.5

Sources:
Clark, David P., and Lonnie D. Russell. Molecular Biology Made Simple and Fun 2nd Edition.. Vienna, IL Cache River Press, 2000.

BIOTECHNOLOGY I
POP BEAD CLONING
21. If the recombinant plasmid were cut with the following enzymes and combinations of enzymes, draw the way the fragments would appear on the finished gel after electrophoresis and staining. Use the map of the recombinant plasmid, rather than the pop beads, to determine the size of all the resulting fragments in each digestion.

1. Kb ladder
2. Pkpk
3. Pkyl
4. Ylyl
5. Pkpk + Pkyl
6. Pkpk + Ylyl
7. Pkyl + Ylyl
8. Uncut recombinant plasmid

12 Kb
6
5
4
3
2
1.5
1.0
0.5

Sources:
Clark, David P., and Lonnie D. Russell. Molecular Biology Made Simple and Fun 2nd Edition.. Vienna, IL Cache River Press, 2000.

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