00:01
Hello students, in this question we have been asked several questions.
00:04
The first one is after doing the of test with the test organism the result showed a green color for the tube with mineral oil and yellow for without oil.
00:25
So what conclusion can you make about this bacterium and name an example species that would produce such results.
00:35
So let us understand the of test shows that the bacterium is fermentative.
00:43
The green color in the tube indicates the bacterium is not oxidizing the carbohydrates while the yellow color indicate that there is carbohydrate fermenting.
01:12
So an example of species that would produce such result is escherichia coli.
01:18
Coming to the second question what is the basis of the catalase test and how do you identify a facultative anaerobe from an aerotolerant.
01:34
So facultative anaerobe from an aerotolerant using the test.
01:52
So the basis is to determine the presence of the enzyme catalase.
01:59
Now catalase breaks down hydrogen peroxide into water and oxygen which helps to protect the bacteria from oxidative damage and to identify a facultative anaerobe from an aerotolerant anaerobe you would look for the production of bubbles when hydrogen peroxide is added to the bacterial culture.
02:27
Facultative anaerobe will produce bubbles but the aerotolerant will not produce bubbles...