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Hello everyone.
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Here in this question we have to tell the results of testing pseudomonas fluorescence with the following tests.
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Okay, so let us first consider our first test that is the triple sugar iron agar broth test.
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Okay, so now this test it is used to detect the gram negative bacteria.
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Okay, now the triple sugar ion agar broth test.
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Test, it determines the carbohydrate fermentation, that is how much the organism can ferment the carbohydrates, that is the three sugars.
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Now for this, three sugars are used which are glucose, lactose, and sucrose.
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An indicator for this is the phenol red.
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Okay.
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Okay.
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Now, the carbohydrate, it would be detected or the carbohydrate fermentation, it would be detected by the presence of gas or there would be a visible color change.
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Okay, this color change is due to the acid production.
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And initially, the color would be red, but if the carbohydrate fermentation has took place, the color change would.
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Be from red to yellow.
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Okay.
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Now in case of pseudomonas fluorescence, it does not ferment.
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It does not ferment any of the three sugar.
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Hence the solution would be alkaline and since no acid is produced, the final color would be red.
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Okay.
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Now the triple sugar ion agar broth test, it also determines the formation of hydrogen sulfide.
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Okay? but in case of hydrogen sulfide, what happens is it is detected by a black ppd, that is, precipitation at the base of the test tube.
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Okay? now the pseudermona fluorescence, it does not produce hydrogen sulfide.
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Okay.
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Now the second test is the indole test.
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Okay.
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Now what does the indole test do is that it tests the ability of the bacteria to convert tryptophan to indole...