Nitrogenous waste in the form of ammonia is a byproduct generated during amino acid metabolism that will build up to toxic levels unless it is removed. In organisms including mammals – known as ureotelic organisms – ammonia is converted to urea for safe storage and excretion. As far as carbon usage is concerned, urea is an efficient means by which to remove excess nitrogen, since two nitrogen atoms can be removed using only a single carbon.
(a) In ureotelic organisms, which organ produces the most urea and which organ collects and excretes it? How many reversible and irreversible reactions are in the urea cycle?
(b) In which cellular compartment do each of the reactions of the urea cycle take place, and which metabolites are transported between cellular compartments?
(c) One molecule of urea contains two nitrogen groups. How do each of these nitrogen groups enter the urea cycle? What are the precursors, intermediates and products of each of these nitrogen-acquiring reactions?