(2 pts) You want to introduce a human gene with two introns into a bacterial host in hopes of producing a large amount of the human gene product. Should you use the genomic DNA or the cDNA? Why?
Added by Catherine C.
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Genomic DNA contains all the genetic information of an organism, including introns, exons, and non-coding regions. cDNA, on the other hand, is synthesized from mRNA and lacks introns, containing only the coding regions (exons) of a gene. Show more…
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If you wanted to express a human gene in bacteria, you would most likely use a. a piece of genomic DNA containing the human gene of interest. b. a cDNA made from the mRNA coding the gene of interest. c. STR analysis. d. CRISPR/Cas9 to transform the bacteria.
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If you wanted a bacterial cell to make a protein from a human gene, how would you amplify the human gene? (Hint: Look up RNA processing in eukaryotic cells) - Use PCR to amplify the gene of interest. - Use reverse transcriptase to make a cDNA of mRNA from the gene of interest, then use PCR to amplify the cDNA. - Use RdRP to make a cDNA of mRNA from the gene of interest, then use PCR to amplify the cDNA.
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You have an oligonucleotide probe that hybridizes to part of gene A from eukaryotic cell. In order to work with it, you need to make a DNA library. You have the choice of making either a genomic DNA library or a cDNA (COPY DNA) library made from mRNA isolated from the cell that has been reverse transcribed back to DNA. Indicate whether a cDNA library or a genomic library is more appropriate for use in the following applications. Explain your answers. A cDNA library will be more appropriate if gene A is alternatively spliced and you want to see which splice products the cell actually produces. If you want to express gene A in bacteria to produce lots of protein, a genomic DNA library would be more suitable.
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