24 4 points Which of the following is TRUE regarding cDNA libraries? They represent the mRNA of a cell or tissue. They can be screened with DNA probes as well as antibody probes. There is reduced information content. E. coli can translate the mRNA into its corresponding protein. All of the above.
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Step 1: cDNA libraries are collections of DNA sequences that are complementary to mRNA molecules. Show more…
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You have an oligonucleotide probe that hybridizes to part of gene A from eukaryotic cell. In order to work with it, you need to make a DNA library. You have the choice of making either a genomic DNA library or a cDNA (COPY DNA) library made from mRNA isolated from the cell that has been reverse transcribed back to DNA. Indicate whether a cDNA library or a genomic library is more appropriate for use in the following applications. Explain your answers. A cDNA library will be more appropriate if gene A is alternatively spliced and you want to see which splice products the cell actually produces. If you want to express gene A in bacteria to produce lots of protein, a genomic DNA library would be more suitable.
Madhur L.
You plan to construct a mouse genomic DNA library, using the plasmid pUC19 shown below as cloning vector. The mouse DNA has already been purified, and digested with the restriction enzyme BamHI. a) How does the construction of a genomic DNA library differ from that of a cDNA library? b) Give one advantage and one disadvantage of having constructed a mouse genomic library (rather than a cDNA library). c) You have constructed the mouse genomic library by cloning into the BamHI site of pUC19, and transformation into E. coli. Explain how you would use a combination of antibiotic and colour selection to identify colonies containing potential genomic clones or recombinant plasmids. d) You want to select a clone from your genomic library that contains gene A. It is known that gene A encodes protein A, with .... His – Cys – Met – Ser – Arg – Phe ..... as part of its amino acid sequence. You wish to make a set of 18 bp DNA probes to screen your DNA library with. i) Considering the degeneracy of the genetic code, how many possible different DNA sequences could encode this amino acid sequence? ii) Give the DNA sequence of any one probe that would be suitable to use. iii) You find that this probe you have designed does not detect any colonies in the genomic library, however it successfully binds to colonies from a cDNA library prepared from the same mouse. Give a possible explanation for this observation.
Sri K.
Which of the following statement is true about cDNA library? cDNA libraries contain DNA copies of mRNA expressed in the sample tissue cDNA libraries contain DNA representing the entire genome. cDNA library would have the greater sequence diversity than genomic library. multiple cDNA libraries would be overlapping completely.
Shveta P.
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