3. You are studying the pathway for nucleotide development in yeast. You perform a mutational screen that identifies a large number of mutants that are unable to synthesize the nucleotide uracil. You have already done complementation tests with these, and have identified 7 complementation groups. You are now interested in putting together a pathway for uracil biosynthesis. You have found several compounds which are in the URA pathway, and try to grow your mutants on them. OPRT CPSII DHO OMPDC ACT DHODH Ura1 + - + + - + Ura2 - - - - - - Ura3 + - + + + + Ura4 + + + + + + Ura5 - - + + - - Ura6 + - - + - + Ura7 + - - + - - Draw the Uracil biosynthesis pathway, including the compounds and the mutants. Ura4 Ura3 Ura1 Ura6 Ura7 Ura5 Ura2 $\rightarrow$CPSII$\rightarrow$ACT$\rightarrow$DHO$\rightarrow$DHODH$\rightarrow$OPRT$\rightarrow$OMPOC$\rightarrow$Growth! 4 You are studying col
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These mutants are Ura1, Ura2, Ura3, Ura4, Ura5, Ura6, and Ura7. Show more…
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1. A graduate student was interested in how an alkylating agent would affect the sole guanine in the sequence of gene X after a round of replication. The sequence of gene X in her yeast colonies was 5’ TATTACGTA 3’ before treatment. Which of the following sequences would reasonably be expected following treatment with the graduate student’s chemical? a. 5’ TATTACUTA 3’ b. 5’ TATTACTTA 3’ c. 5’ TATTACGTA 3’ (no change) d. 5’ TATTACATA 3’ e. 5’ TATTACCTA 3’ 2. A DNA sequence is mutated such that it now contains hypoxanthine in the place of an adenine. Which of the following enzymes would be involved in the repair of this mutation? a. Glycosylase b. Alkyltransferase c. UrvA-UrvB complex d. Ku heterodimer e. Photolyase
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The following nucleotide sequence is found on the template strand of DNA. First, determine the amino acids of the protein encoded by this sequence by using the genetic code provided in Figure 15.10. Then give the altered amino acid sequence of the protein that will be found in each of the following mutations: (EQUATION CAN'T COPY) a. Mutant 1: A transition at nucleotide 11 b. Mutant 2: A transition at nucleotide 13 c. Mutant 3: A one-nucleotide deletion at nucleotide 7 d. Mutant 4: A T S A transversion at nucleotide 15 e. Mutant 5: An addition of TGG after nucleotide 6 f. Mutant 6: A transition at nucleotide 9
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