3. (4 points total) Your friend decides to perform an experiment similar to the Hershey-Chase experiment. They prepare the following four infections: i) they label bacteriophage viral protein with radioactive sulfur, ii) they label bacteriophage viral DNA with radioactive phosphorus, iii) they label bacteriophage viral protein with radioactive sulfur and viral DNA with radioactive phosphorus, and iv) they treat bacteriophage with buffer. They then infect separate E. coli with each treatment group, blend/separate the virus from the E.coli with a Vitamix5200, centrifuge to separate the pellet and supernatant, and measure radioactivity in the viral progeny. Circle the graph below that most likely reflects the expected results and explain why you made your selection. A 20 10 i ii iii iv Samples B 20 10 i ii iii iv Samples C 20 10 i ii iii iv Samples D 20 10 i ii iii iv Samples
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When the virus infects the E. coli, the viral DNA will be injected into the host cell, while the protein coat will remain outside. Show more…
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Your friend decides to perform an experiment similar to the Hershey-Chase experiment. They prepare the following four infections: i) they label bacteriophage viral protein with radioactive sulfur; ii) they label bacteriophage viral DNA with radioactive phosphorus; iii) they label bacteriophage viral protein with radioactive sulfur and viral DNA with radioactive phosphorus; and iv) they treat bacteriophage with buffer. They then infect separate E. coli with each treatment group, blend/separate the virus from the E.coli with a Vitamix5200, centrifuge to separate the pellet and supernatant, and measure radioactivity in the viral progeny. Circle the graph below that most likely reflects the expected results and explain why you made your selection.
Adi S.
In a classic 1952 experiment, biologists Alfred Hershey and Martha Chase labeled two batches of bacteriophages, one with radioactive sulfur (which only tags protein) and the other with radioactive phosphorus (which only tags DNA). In separate test tubes, they allowedeach batch of phages to bind to nonradioactive bacteria and inject its DNA. After a few minutes, they separated the bacterial cells from the viral parts that remained outside the bacterial cells and measured the radioactivity of both portions. What results do you think they obtained? How would these results help them to determine which viral component-DNA or protein-was the infectious portion?
Sri K.
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