4. Calculate the concentration and purity of the following DNA samples. Comment on the purity of each sample. SAMPLE OD<sub>260</sub> OD<sub>280</sub> DNA Concentration DNA purity 10 µl dissolved in 1000 µl of water 0.213 0.210 5 µl dissolved in 500 µl of water 0.146 0.086 20 µl dissolved in 100 µl of water 0.178 0.09
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To calculate the DNA concentration, we need to use the formula: Concentration (ng/ul) = OD260 x dilution factor x conversion factor The dilution factor is the ratio of the final volume to the initial volume, and the conversion factor is the factor that converts Show more…
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