Question

Calculate the estimated concentration and yield of a 1 mL DNA sample, if the absorbance at 260nm is 0.862 when you dilute it 1:200 and test it using the spectrophotometer (4 points). Formulae for calculation: Concentration (ng/µL) = Abs@260 × 50 × dilution factor DNA yield (µg) = DNA concentration x total sample volume (mL)

Name: calculate the estimated concentration and yield of a 1 ml dna sample if absorbance at 260nm is 0862 when you dilute it 1200 and test it using the spectrophotometer 4 points formulae for calc 38557
Uploaded: 2022-11-25T16:08:37-08:00
Duration: 2 min 13 s
Channel: Jenny Wu
Description: calculate the estimated concentration and yield of a 1 ml dna sample if absorbance at 260nm is 0862 when you dilute it 1200 and test it using the spectrophotometer 4 points formulae for calc 38557

          Calculate the estimated concentration and yield of a 1 mL DNA sample, if the absorbance at 260nm is 0.862 when you dilute it 1:200 and test it using the spectrophotometer (4 points).

Formulae for calculation: 
Concentration (ng/µL) = Abs@260 × 50 × dilution factor
DNA yield (µg) = DNA concentration x total sample volume (mL)

Added by Albert C.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Jenny Wu

11/25/2022

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862 - Dilution factor = 1:200 - Total sample volume = 1 mL Show more…

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Calculate the estimated concentration and yield of a 1 mL DNA sample, if the absorbance at 260nm is 0.862 when you dilute it 1:200 and test it using the spectrophotometer (4 points). Formulae for calculation: Concentration (ng/µL) = Abs@260 × 50 × dilution factor DNA yield (µg) = DNA concentration x total sample volume (mL)