A DNA preparation diluted 1/100 yields an absorbance reading of 0.25 at 260 nm. What is the total yield of DNA if you have 3.0 mL? Calculate the total yield of the DNA prep and fill in the blank. The total DNA yield is _______.
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Step 1
Given: Absorbance reading at 260 nm = 0.25 Dilution factor = 1/100 Total volume of sample = 3.0 mL Absorbance = 0.25 Absorbance of 1/100 dilution = 0.25 * 100 = 25 Concentration of DNA in the sample = Absorbance * Absorbance unit * Dilution factor Concentration = Show more…
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