6. (6 points) Match the following components of the CRISPR/Cas9 bacterial immune system with the appropriate description. Each answer is used only once. A. crRNA B. Protospacer C. Spacer D. CRISPR E. Cas9 F. PAM ______ The specific sequence of an invading phage DNA that is cut by the Cas9 endonuclease. ______ Is responsible for the recognition of foreign DNA upon subsequent infection. ______ A sequence in the bacterial genome that is identical to part of the invading phage DNA. ______ The enzyme that destroys phage DNA upon subsequent infection by a foreign DNA. ______ Repeat sequences in the bacterial genome that separate copies of phage DNA sequences. ______ Is the feature on the invading DNA that is required in order for Cas9 to cut.
Added by Heather M.
Close
Step 1
The specific sequence of an invading phage DNA that is cut by the Cas9 endonuclease: This is the Protospacer. So, A = \boxed{\text{Protospacer}}. Show more…
Show all steps
Your feedback will help us improve your experience
Shaiju T and 68 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Question 5: (12 marks) The CRISPR-Cas9 genome editing technology arose from the Nobel Prize-winning research by Dr. Emmanuelle Charpentier, director at the Max Planck Institute for Infection Biology, and Dr. Jennifer Doudna, a biochemist at the University of California Berkeley. They were able to adapt an obscure bacterial immune mechanism into a tool that can easily and cheaply edit the genome of nearly any organism. Explain how the CRISPR-Cas9 system can do the following and describe what additional components, if any, are needed to attain each outcome: a) Disrupt a gene b) Repair a gene c) Disrupt more than one gene d) Enhance or block expression of a gene
Adi S.
Which answer best describes the natural role of CRISPR in bacteria? Answer choice: a) Adaptive immune system, b) Innate immune system Which molecule gives the CRISPR technique its incredible flexibility for use as a gene editing tool? Answer choice a) RNA b) Cas9 c) tracrRNA This molecule can be used to very sensitively detect RNA viruses: Answer choice a) Cas9, b) Cas13, c) Reverse transcriptase In Prime Editing, this molecule is fused to Cas9 to allow DNA base editing without creating double-stranded DNA breaks:
Krishna G.
In a strain of bacteria, a mutation changes the sequence of repeat 2 in the CRISPR gene (see figure). How will this affect bacterial immunity? This is an analysis question because you have to break the CRISPR-Cas system into its component pieces to understand how a mutation will affect its function. A) The bacteria will have no defense against the first infection with any bacteriophage. B) The bacteria not have adaptive immunity against any second infections. C) The bacteria will have adaptive immunity against second infection with bacteriophage A, but not B, C, or D. D) The bacteria will have adaptive immunity against second infection with bacteriophage A, C, and D, but not B.
Shaiju T.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD